Amido black 10B
Names | |
---|---|
IUPAC name Sodium 4-amino-5-hydroxy-3-((E)-(4-nitrophenyl)diazenyl)-6-((E)-phenyldiazenyl)naphthalene-2,7-disulfonate | |
Other names 4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalene disulfonic acid, disodium salt Amidoschwarz Naphthol blue black Acid Black 1 Acidal Black 10B Acidal Navy Blue 3BR Naphthalene Black 10B Buffalo Black NBR C.I. 20470 | |
Identifiers | |
CAS Number |
|
3D model (JSmol) |
|
ChEBI |
|
ChemSpider |
|
ECHA InfoCard | 100.012.640 |
EC Number |
|
PubChem CID |
|
UNII |
|
CompTox Dashboard (EPA) |
|
InChI
| |
| |
Properties | |
Chemical formula | C22H14N6Na2O9S2 |
Molar mass | 616.49 g·mol−1 |
Appearance | Dark red to black |
Solubility in water | ~ 30 g/L at 20 °C (68 °F) |
Hazards | |
GHS labelling: | |
Pictograms | |
Warning | |
NFPA 704 (fire diamond) | 2 0 0 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). N verify (what is YN ?) Infobox references |
Chemical compound
Amido black 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, such as the western blot.[2] It is also used in criminal investigations to detect blood present with latent fingerprints - it stains the proteins in blood a blue-black color.[3] Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.
See also
References
- ^ "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017.
- ^ Kurien, B. T., & Scofield, R. H. (2015). Western Blotting: An Introduction. In B. T. Kurien & R. H. Scofield (Eds.), Western Blotting: Methods and Protocols (pp. 17–30). Springer. ISBN 978-1-4939-2694-7
- ^ Bossers, L. C. A. M., Roux, C., Bell, M., & McDonagh, A. M. (2011). Methods for the enhancement of fingermarks in blood. Forensic Science International, 210(1), 1–11. doi:10.1016/j.forsciint.2011.04.006
External links
- MSDS at Oxford University
- v
- t
- e
EAATsTooltip Excitatory amino acid transporters |
|
---|---|
vGluTsTooltip Vesicular glutamate transporters |
|
GAHTooltip Glutamine aminohydrolase (glutaminase) |
|
---|---|
ASTTooltip Aspartate aminotransferase |
|
ALTTooltip Alanine aminotransferase |
|
GDHTooltip Glutamate dehydrogenase |
|
GSTooltip Glutamine synthetase | |
GADTooltip Glutamate decarboxylase |
|
See also: Receptor/signaling modulators • Ionotropic glutamate receptor modulators • Metabotropic glutamate receptor modulators • GABA metabolism and transport modulators